{"id":10135,"date":"2022-03-28T10:38:28","date_gmt":"2022-03-28T09:38:28","guid":{"rendered":"https:\/\/fei-online.com\/?p=10135"},"modified":"2022-03-28T10:38:28","modified_gmt":"2022-03-28T09:38:28","slug":"headspace-sampling-using-hisorb-high-capacity-sorptive-extraction-for-flavour-profiling-of-foods-and-beverages","status":"publish","type":"post","link":"https:\/\/fei-online.com\/headspace-sampling-using-hisorb-high-capacity-sorptive-extraction-for-flavour-profiling-of-foods-and-beverages\/","title":{"rendered":"Headspace sampling  using HiSorb high-capacity sorptive extraction for flavour profiling of foods  and  beverages"},"content":{"rendered":"<div id='av_section_1'  class='avia-section main_color avia-section-default avia-no-border-styling  avia-bg-style-scroll  avia-builder-el-0  avia-builder-el-no-sibling   container_wrap fullsize' style=' '  ><div class='container' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-10135'><div class='entry-content-wrapper clearfix'>\n<div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding first  avia-builder-el-1  el_before_av_three_fifth  avia-builder-el-first  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\"><div id=\"ads-slider-28\" class=\"custom-slider ads-slider-1774087011 ads-slider\"><ul><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/goto\/?308404\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/308404.jpg\" alt=\"Free from food\" title=\"Free from food\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=302805\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/302805.jpg\" alt=\"LUBRIPLATE\" title=\"LUBRIPLATE\" border=\"0\" width=\"120\" height=\"600\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><div style=\"margin-left: auto; margin-right: auto; text-align: center; \" id=\"ads-1065588327\"><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=297426\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" width=\"100%\" height=\"auto\" src=\"https:\/\/clixtrac.com\/banner\/297426.jpg\" alt=\"Click here for more information!\" title=\"Click here for more information!\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/div><\/li><\/ul><\/div><script>( window.advanced_ads_ready || jQuery( document ).ready ).call( null, function() {var $adsslider1774087011 = jQuery( \".ads-slider-1774087011\" );$adsslider1774087011.on( \"unslider.ready\", function() { jQuery( \"div.custom-slider ul li\" ).css( \"display\", \"block\" ); });$adsslider1774087011.unslider({ delay:5000, autoplay:true, nav:false, arrows:false, infinite:true });$adsslider1774087011.on(\"mouseover\", function(){$adsslider1774087011.unslider(\"stop\");}).on(\"mouseout\", function() {$adsslider1774087011.unslider(\"start\");});});<\/script><\/p>\n<\/div><\/section><\/div><div class=\"flex_column av_three_fifth  flex_column_div av-zero-column-padding   avia-builder-el-3  el_after_av_one_fifth  el_before_av_one_fifth  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\"><\/p>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><header class=\"entry-content-header\"><h1 class=\"post-title entry-title\" itemprop=\"headline\">Headspace sampling  using HiSorb high-capacity sorptive extraction for flavour profiling of foods  and  beverages<\/h1><span class=\"post-meta-infos\">\/ <span class=\"blog-categories minor-meta\">in <a href=\"https:\/\/fei-online.com\/category\/featured-articles\/\" rel=\"tag\">Featured Articles<\/a> <\/span><\/span><\/header>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><h4><strong>Summary<\/strong><\/h4>\n<h4>This study demonstrates the performance of HiSorb\u2122 high-capacity sorptive extraction probes for flavour profiling of two samples \u2013 cider and potato snacks (crisps) \u2013 contrasting the unflavoured product with those that have had flavourings added. HiSorb is a solvent-free technique utilising a high- capacity sorptive phase to ensure good extraction efficiency and is readily applicable to both headspace and immersive sampling. In conjunction with the Centri\u00ae extraction and enrichment platform, HiSorb is fully automatable.<\/h4>\n<p><!--more--><\/p>\n<h4><strong>Introduction<\/strong><\/h4>\n<p>Analysis of the volatile organic compound (VOC) content of food- stuffs is an important tool in the food and beverage industry, with applications in research and development, quality control, shelf-life assessment and even detection of food fraud. HiSorb high-capacity sorptive extraction probes offer a highly sensitive, robust and fully automatable flavour profiling solution, and have provided excellent results in gas chromatography\u2013mass spectrometry (GC\u2013MS)-based analysis of foodstuffs.1,2,3<\/p>\n<p>The robust design of HiSorb probes allows for flexibility in sampling method. Probes may be suspended above a sample to extract VOCs from the headspace or immersed directly into a liquid sample so that the sorptive phase is in direct contact with the sample matrix (Figure 1). By removing the requirement for VOCs to partition into the headspace first, immersive sampling can improve extraction efficiency for some compounds.<\/p>\n<p>Following sorptive extraction, HiSorb probes must be desorbed with heat so that analytes can be transferred to the analytical system. The Centri extraction and enrichment platform is the ideal option for coupling HiSorb extraction with GC analysis, fully automating all aspects of sampling and incorporating Markes\u2019 leading focusing trap technology (Figure 2) for hands-free, high-throughput analysis with optimal chromatography. Alternatively, HiSorb probes can be used with an off-line agitator for sample extraction. Probes fit inside industry-standard thermal desorption tubes and can be desorbed with dedicated thermal desorption instruments such as Markes\u2019 TD100-xr\u2122.<\/p>\n<p>Here, we demonstrate the ability of HiSorb probes to provide comprehensive flavour profiles of foodstuffs, by comparing the VOCs extracted from unflavoured products with those extracted from products after addition of a flavour mix. We show extraction from the headspace of a solid sample (potato snacks) and a liquid sample (cider), detecting a broad range of compounds with both techniques.<\/p>\n<h4><strong>Experimental<\/strong><\/h4>\n<p>Sample preparation:<br \/>\nCrisps: 3g, ground<br \/>\nCider: 10 mL undiluted cider<\/p>\n<p>Sampling: Instrument:<br \/>\nHiSorb Agitator (U-HSAG-20)<br \/>\nHiSorb probes: PDMS, short length, inert-coated (part no. H1-AXABC)<br \/>\nIncubation: 37\u00b0C for 30 min<\/p>\n<p>Thermal desorption:<br \/>\nInstrument: TD100-xr<br \/>\nFlow path: 180\u00b0C<br \/>\nProbe desorption: 270\u00b0C for 10 min<br \/>\nTrap: \u2018Material emissions\u2019 (part no. U-T12ME-2S)<br \/>\nTrap temperature: 30\u00b0C Trap dry purge: 1 min at 50 mL\/min<br \/>\nTrap desorption: Max heating rate to 290\u00b0C, 3 min<br \/>\nOutlet split: 50 mL\/min (26:1)<\/p>\n<p>GC:<br \/>\nColumn: DB-5\u2122 \u2013 30 m x 0.25 mm i.d. x 0.25 mm film thickness<br \/>\nColumn flow: 2 mL\/min<br \/>\nOven ramp: 40\u00b0C (3 min), 10\u00b0C \/ min to 310\u00b0C (10 min)<br \/>\nInlet: 200\u00b0C<\/p>\n<p>MS:<br \/>\nMS transfer line: 310\u00b0C<br \/>\nMS source: 250\u00b0C<br \/>\nQuad: 180\u00b0C<br \/>\nScan range: 35\u2013350 m\/z<\/p>\n<h4><strong>Results and discussion<\/strong><\/h4>\n<p><strong>Potato snacks <\/strong><br \/>\nPotato snacks (crisps) were sampled by suspending a HiSorb probe above a dry, homogenised sample for 30 minutes at 37\u00b0C (to emulate the temperature inside the mouth).<\/p>\n<p>Extracted compounds were subsequently analysed by thermal desorption\u2013gas chromatography\u2013mass spectrometry (TD\u2013 GC\u2013MS). Data processing was carried out using ChromSpace\u00ae (SepSolve Analytical).<\/p>\n<p>Figure 3 shows the total ion chromatograms (TICs) produced from samples of unflavoured crisps (A) and crisps after addition of a cheese flavour mix (B). Peaks are labelled if they were confidently identified and with a NIST match factor (MF) &gt; 750. The 12 peaks labelled in A represent analytes detected in both samples, while the eight peaks labelled in B indicate analytes found only in the flavoured sample (bold labelling). Compounds unique to B typically conferred cheesy- or dairy-like flavours, as expected for compounds in a cheese flavour mix. This highlights the power of HiSorb to extract the important flavour constituents and distinguish between similar, yet distinct products.<\/p>\n<p><strong>Cider <\/strong><br \/>\nFor liquid samples, HiSorb probes are compatible with extraction from the headspace or via direct immersion into the matrix. Here, cider was sampled by suspending a probe in the headspace above a 10-mL undiluted sample, with parameters and downstream analysis as described for the potato snack samples.<\/p>\n<p>Figure 4 compares TIC profiles of an unflavoured cider (A) with cider enriched with a fruit flavouring mix (B). 52 peaks had NIST MF &gt; 750, of which 39 are shown. Substantial differences were observed between the two sample types, with 27 compounds either newly present (bold labels) or present at substantially higher abundance (underlined labels) in the flavoured sample. Notably, most of these compounds had sweet, fruity and\/or herbal flavour characters, as expected from additives in a fruit flavouring mix. The 13 compounds not shown in Figure 4 all eluted before the retention window shown and were all present at approximately equal concentrations in the two cider samples.<\/p>\n<p>In conclusion, we have demonstrated good performance of HiSorb extraction and enrichment probes, combined with TD\u2013GC\u2013MS, for the analysis and comparison of flavoured and unflavoured varieties of cider and potato snacks. The extensive flavour profiles generated highlight robust detection of these flavour-active compounds in both solid (potato snack) and liquid (cider) matrices. In addition to this, the high phase loading of HiSorb can provide improved extraction efficiency relative to conventional extraction methods, such as solid- phase microextraction (SPME, ~0.5\u20131 \u03bcL phase loading), which are typically used for flavour and aroma profiling.<\/p>\n<p><em>For more information: email: <a href=\"mailto:enquiries@markes.com\">enquiries@markes.com<\/a> \/ visit: <a href=\"http:\/\/www.markes.com\">www.markes.com<\/a><\/em><\/p>\n<\/div><\/section><br \/>\n<div  class='avia-image-container  av-styling-    avia-builder-el-7  el_after_av_textblock  el_before_av_textblock  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/fei-online.com\/wp-content\/uploads\/sites\/4\/2022\/03\/hiSorb-Fig_1.jpg' alt='' title='hiSorb-Fig_1' height=\"868\" width=\"1868\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><em><b>Figure 1: Headspace (left) and immersive (right) sampling with HiSorb probes.<\/b><\/em><\/p>\n<\/div><\/section><br \/>\n<div  class='avia-image-container  av-styling-    avia-builder-el-9  el_after_av_textblock  el_before_av_textblock  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/fei-online.com\/wp-content\/uploads\/sites\/4\/2022\/03\/hisrob-fig-2.jpg' alt='' title='hisrob-fig-2' height=\"1178\" width=\"1602\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><em><b>Figure 2: HiSorb extraction workflows using Markes\u2019 thermal desorbers. Bottom: offline, manual sample extraction using a HiSorb agitator, followed by probe desorption inside an empty TD tube. Top: fully automated workflow on Centri, from sampling to GC injection<\/b><\/em><\/p>\n<\/div><\/section><br \/>\n<div  class='avia-image-container  av-styling-    avia-builder-el-11  el_after_av_textblock  el_before_av_textblock  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/fei-online.com\/wp-content\/uploads\/sites\/4\/2022\/03\/hisorb-fig-3.jpg' alt='' title='hisorb-fig-3' height=\"1110\" width=\"1942\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><em><b>Figure 3: Flavour profiles of unflavoured (A) and cheese flavoured (B) potato snacks, obtained by HiSorb extraction. Compounds given in bold were found only in the cheese flavoured sample.<\/b><\/em><\/p>\n<\/div><\/section><br \/>\n<div  class='avia-image-container  av-styling-    avia-builder-el-13  el_after_av_textblock  el_before_av_textblock  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/fei-online.com\/wp-content\/uploads\/sites\/4\/2022\/03\/hisorb-fig-4.jpg' alt='' title='hisorb-fig-4' height=\"1260\" width=\"1982\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><em><b>Figure 4: Flavour profiles of (A) unflavoured and (B) fruit flavoured cider, obtained by HiSorb. 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HiSorb is a solvent-free technique utilising a high- capacity sorptive phase to ensure good extraction efficiency and is readily [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10140,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[26],"tags":[],"_links":{"self":[{"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/posts\/10135"}],"collection":[{"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/comments?post=10135"}],"version-history":[{"count":2,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/posts\/10135\/revisions"}],"predecessor-version":[{"id":10142,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/posts\/10135\/revisions\/10142"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/media\/10140"}],"wp:attachment":[{"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/media?parent=10135"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/categories?post=10135"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fei-online.com\/wp-json\/wp\/v2\/tags?post=10135"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}