{"id":10395,"date":"2022-06-28T09:02:13","date_gmt":"2022-06-28T08:02:13","guid":{"rendered":"https:\/\/fei-online.com\/?p=10395"},"modified":"2022-08-25T09:42:31","modified_gmt":"2022-08-25T08:42:31","slug":"ndma-in-malt-and-beer-2","status":"publish","type":"post","link":"https:\/\/fei-online.com\/ndma-in-malt-and-beer-2\/","title":{"rendered":"NDMA in Malt and Beer"},"content":{"rendered":"<div id='av_section_1'  class='avia-section main_color avia-section-default avia-no-border-styling  avia-bg-style-scroll  avia-builder-el-0  avia-builder-el-no-sibling   container_wrap fullsize' style=' '  ><div class='container' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-10395'><div class='entry-content-wrapper clearfix'>\n<div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding first  avia-builder-el-1  el_before_av_three_fifth  avia-builder-el-first  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\"><div id=\"ads-slider-28\" class=\"custom-slider ads-slider-780933099 ads-slider\"><ul><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/goto\/?308404\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/308404.jpg\" alt=\"Free from food\" title=\"Free from food\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=302805\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/302805.jpg\" alt=\"LUBRIPLATE\" title=\"LUBRIPLATE\" border=\"0\" width=\"120\" height=\"600\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><div style=\"margin-left: auto; margin-right: auto; text-align: center; \" id=\"ads-76964494\"><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=297426\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" width=\"100%\" height=\"auto\" src=\"https:\/\/clixtrac.com\/banner\/297426.jpg\" alt=\"Click here for more information!\" title=\"Click here for more information!\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/div><\/li><\/ul><\/div><script>( window.advanced_ads_ready || jQuery( document ).ready ).call( null, function() {var $adsslider780933099 = jQuery( \".ads-slider-780933099\" );$adsslider780933099.on( \"unslider.ready\", function() { jQuery( \"div.custom-slider ul li\" ).css( \"display\", \"block\" ); });$adsslider780933099.unslider({ delay:5000, autoplay:true, nav:false, arrows:false, infinite:true });$adsslider780933099.on(\"mouseover\", function(){$adsslider780933099.unslider(\"stop\");}).on(\"mouseout\", function() {$adsslider780933099.unslider(\"start\");});});<\/script><\/p>\n<\/div><\/section><\/div><div class=\"flex_column av_three_fifth  flex_column_div av-zero-column-padding   avia-builder-el-3  el_after_av_one_fifth  el_before_av_one_fifth  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\"><\/p>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><header class=\"entry-content-header\"><h1 class=\"post-title entry-title\" itemprop=\"headline\">NDMA in Malt and Beer<\/h1><span class=\"post-meta-infos\">\/ <span class=\"blog-categories minor-meta\">in <a href=\"https:\/\/fei-online.com\/category\/featured-articles\/\" rel=\"tag\">Featured Articles<\/a> <\/span><\/span><\/header>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><strong>NDMA (n-nitrosodimethylamine) in Malt\/Beer<\/strong><\/p>\n<p><!--more--><\/p>\n<p>During the malting process, barley is forced to germinate, after which the grain is dried in kilns. This process freezes the sugar and flavour compounds for the brewing process. During the kiln drying process, nitrosamines may be formed in the grain, which could remain within the extract and still be present in the final brewed product. Many recently used techniques, minimise the formation of nitrosamines., However, low levels of these carcinogenic compounds still remain. Therefore, all malt used in the brewing process needs to be analysed for its nitrosamine content. It is important to monitor the final product as well as the malt, to help regulate the exposure of nitrosamines from the consumed liquid.<\/p>\n<p>The Ellutia 200 Series Gas Chromatograph with an EL-WAX column was utilised alongside the 800 series Thermal Energy Analyser (TEA)<\/p>\n<h4><strong>Malt<\/strong><\/h4>\n<p>Samples were extracted in duplicate. Each replicate had 50 grams of malt ground up and had 100 ml deionised water added. The extract was filtered through a Whatman Grade 1 filter paper and 1 ml of 10 ppm NDPA (n-nitroso di propylamine) internal standard was added to one extract (this generates a 100 ppb NDPA spiked sample). The samples were then made up to 100 ml with deionized water volumetrically. To a vial, 10 ml of extract, 3 grams of sodium chloride and 10 ml of dichloromethane (DCM) was added and shaken for 5 minutes. Then the layers were left to separate for 15 minutes. The lower layer containing DCM was pipetted out into a clean vessel. 10 ml of DCM was added to the extract and the liquid\/liquid extraction step was repeated. After this step, the DCM (final volume approx. 20 ml) was dried using 1 gram of sodium sulphate and then preconcentrated to 1 ml under a nitrogen flow of approx.1 l min-1. A 1 \u00b5l injection of the concentrated DCM was directly analysed.<\/p>\n<p>The identity of the internal standard was confirmed against the 8 component nitrosamine mix standard. This shows that the NDPA internal standard used has the same retention time as the NDPA contained within the standard (figure 1).The peak areas for the spiked sample compared with the standard for NDPA showed good correlation, indicating a good recovery of the internal standard, and thusly, indicating very limited losses of any potential nitrosamines from within the sample during the pre-paration steps. The unspiked sample showed no peaks within the retention times of any of the nitrosamines in this standard mix. As such, this malt sample showed no observable response for NDMA or any other nitrosamines.<\/p>\n<h4><strong>Beer<\/strong><\/h4>\n<p>25 ml of lager was sonicated for 10 minutes to remove any dissolved gases. 10 ml was transferred to a centrifugal tube, 3 grams of sodium chloride was added, followed by 1 mL of DCM and the solution was shaken for 5 minutes. The sample was then left to separate out for 15 minutes. The DCM layer was pipetted into a new vial and 1 gram of sodium sulphate was added to capture any remaining aqueous solution. A 1 \u00b5l injection of the DCM solution was directly analysed.<\/p>\n<p>NDMA detection limits for this instrument have recently been generated and show that &lt;1 ppb NDMA can be seen. For the malt sample the results would be declared as none detected &lt;1 ppb, the beer would also be declared as &lt;1 ppb NDMA, however, there was a response found for NDBA (n-nitrosobutylamine) of 67 ppb.<\/p>\n<\/div><\/section><br \/>\n<div  class='avia-image-container  av-styling-    avia-builder-el-7  el_after_av_textblock  el_before_av_textblock  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/fei-online.com\/wp-content\/uploads\/sites\/4\/2022\/06\/shutterstock_212771023-1000x1500.jpg' alt='' title='Glass,Of,Beer,Isolated.,Clipping,Path' height=\"1500\" width=\"1000\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><em>This App Note is provided by:<\/em><\/p>\n<p>Ellutia Chromatography Solutions.<br \/>\n<a title=\"http:\/\/www.ellutia.com\/\" href=\"http:\/\/www.ellutia.com\">www.ellutia.com<\/a><\/p>\n<p>For more information and to view the Ordering Guide, visit here:<br \/>\n<a href=\"https:\/\/bit.ly\/3NfR0SQ\">https:\/\/bit.ly\/3NfR0SQ<\/a><\/p>\n<\/div><\/section><br \/>\n<div  class='av-social-sharing-box  avia-builder-el-9  el_after_av_textblock  avia-builder-el-last  av-social-sharing-box-minimal '><div class='av-share-box'><h5 class='av-share-link-description av-no-toc '>Share this<\/h5><ul class='av-share-box-list noLightbox'><li class='av-share-link av-social-link-facebook' ><a target=\"_blank\" aria-label=\"Share on Facebook\" href='https:\/\/www.facebook.com\/sharer.php?u=https:\/\/fei-online.com\/ndma-in-malt-and-beer-2\/&#038;t=NDMA%20in%20Malt%20and%20Beer' aria-hidden='false' data-av_icon='\ue8f3' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Facebook' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Facebook<\/span><\/a><\/li><li class='av-share-link av-social-link-twitter' ><a target=\"_blank\" aria-label=\"Share on Twitter\" href='https:\/\/twitter.com\/share?text=NDMA%20in%20Malt%20and%20Beer&#038;url=https:\/\/fei-online.com\/?p=10395' aria-hidden='false' data-av_icon='\ue8f1' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Twitter' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Twitter<\/span><\/a><\/li><li class='av-share-link av-social-link-whatsapp' ><a target=\"_blank\" aria-label=\"Share on WhatsApp\" href='https:\/\/api.whatsapp.com\/send?text=https:\/\/fei-online.com\/ndma-in-malt-and-beer-2\/' aria-hidden='false' data-av_icon='\uf232' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on WhatsApp' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on WhatsApp<\/span><\/a><\/li><li class='av-share-link av-social-link-linkedin' ><a target=\"_blank\" aria-label=\"Share on LinkedIn\" href='https:\/\/linkedin.com\/shareArticle?mini=true&#038;title=NDMA%20in%20Malt%20and%20Beer&#038;url=https:\/\/fei-online.com\/ndma-in-malt-and-beer-2\/' aria-hidden='false' data-av_icon='\ue8fc' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on LinkedIn' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on LinkedIn<\/span><\/a><\/li><li class='av-share-link av-social-link-mail' ><a  aria-label=\"Share by Mail\" href='mailto:?subject=NDMA%20in%20Malt%20and%20Beer&#038;body=https:\/\/fei-online.com\/ndma-in-malt-and-beer-2\/' aria-hidden='false' data-av_icon='\ue805' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share by Mail'><span class='avia_hidden_link_text'>Share by Mail<\/span><\/a><\/li><\/ul><\/div><\/div><\/p><\/div><div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding   avia-builder-el-10  el_after_av_three_fifth  avia-builder-el-last  \" style='border-radius:0px; 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