{"id":9645,"date":"2021-04-28T14:09:07","date_gmt":"2021-04-28T13:09:07","guid":{"rendered":"https:\/\/fei-online.com\/?p=9645"},"modified":"2021-05-05T14:18:04","modified_gmt":"2021-05-05T13:18:04","slug":"high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii","status":"publish","type":"post","link":"https:\/\/fei-online.com\/high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii\/","title":{"rendered":"High speed analysis of monosaccharides and disaccharides in soft drinks by ELSD-LT III"},"content":{"rendered":"<div id='av_section_1'  class='avia-section main_color avia-section-default avia-no-border-styling  avia-bg-style-scroll  avia-builder-el-0  avia-builder-el-no-sibling   container_wrap fullsize' style=' '  ><div class='container' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-9645'><div class='entry-content-wrapper clearfix'>\n<div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding first  avia-builder-el-1  el_before_av_three_fifth  avia-builder-el-first  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center;\"><div id=\"ads-slider-28\" class=\"custom-slider ads-slider-764262259 ads-slider\"><ul><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/goto\/?308404\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/308404.jpg\" alt=\"Free from food\" title=\"Free from food\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=302805\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" src=\"https:\/\/clixtrac.com\/banner\/302805.jpg\" alt=\"LUBRIPLATE\" title=\"LUBRIPLATE\" border=\"0\" width=\"120\" height=\"600\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/li><li><div style=\"margin-left: auto; margin-right: auto; text-align: center; \" id=\"ads-1790906712\"><!--Begin ClixTrac.com Banner Code -->\r\n<a href=\"https:\/\/clixtrac.com\/banner\/click.php?banner=297426\" target=\"_blank\" rel=\"noopener\">\r\n<img decoding=\"async\" width=\"100%\" height=\"auto\" src=\"https:\/\/clixtrac.com\/banner\/297426.jpg\" alt=\"Click here for more information!\" title=\"Click here for more information!\" border=\"0\" \/><\/a>\r\n<!--End ClixTrac.com Banner Code --><\/div><\/li><\/ul><\/div><script>( window.advanced_ads_ready || jQuery( document ).ready ).call( null, function() {var $adsslider764262259 = jQuery( \".ads-slider-764262259\" );$adsslider764262259.on( \"unslider.ready\", function() { jQuery( \"div.custom-slider ul li\" ).css( \"display\", \"block\" ); });$adsslider764262259.unslider({ delay:5000, autoplay:true, nav:false, arrows:false, infinite:true });$adsslider764262259.on(\"mouseover\", function(){$adsslider764262259.unslider(\"stop\");}).on(\"mouseout\", function() {$adsslider764262259.unslider(\"start\");});});<\/script><\/p>\n<\/div><\/section><\/div><div class=\"flex_column av_three_fifth  flex_column_div av-zero-column-padding   avia-builder-el-3  el_after_av_one_fifth  el_before_av_one_fifth  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center;\"><\/p>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><header class=\"entry-content-header\"><h1 class=\"post-title entry-title\" itemprop=\"headline\">High speed analysis of monosaccharides and disaccharides in soft drinks by ELSD-LT III<\/h1><span class=\"post-meta-infos\">\/ <span class=\"blog-categories minor-meta\">in <a href=\"https:\/\/fei-online.com\/category\/featured-articles\/\" rel=\"tag\">Featured Articles<\/a> <\/span><\/span><\/header>\n<\/div><\/section><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><strong>Saccharides show a very narrow UV absorption wavelength range, from 190 nm to 195 nm since the hydroxyl group is the only major functional group showing UV absorption in the structure. Hence a refractive index detector (RID) is commonly used for this analysis.<\/strong><\/p>\n<p><!--more--><\/p>\n<p>However, gradient elution cannot be used with RID because the baseline drifting derived from the change of mobile phase composition during gradient elution is practically unacceptable. So, RID is not suitable for simultaneous separation of compounds that show widely different retention behaviors due to expected long analysis time without gradient elution. Evaporative light scattering detector (ELSD) is a universal detector that detects light scattering from the target compounds after nebulizing and evaporating of the mobile phase. ELSD provides reduced analysis time and simultaneous separation of compounds that show widely different retention due to its applicability to gradient elution. Monosaccharides and disaccharides are analyzed mainly by anion-exchange chromatography or ligand exchange chromatography. However, it takes a long time to analyze. So, in this article, monosaccharides and disaccharides were separated in short time by hydrophilic interaction chromatography (HILIC) and were detected by ELSD.<\/p>\n<p>\u201cWide function\u201d, a new feature of ELSD-LT III used in this article, automatically optimizes a parameter related to sensitivity and a single method file can be used for data acquisition regardless of sample concentration, from low to high. Here, high speed analysis of monosaccharides and disaccharides in soft drinks were carried out by ELSD-LT III.<\/p>\n<h3>Analysis method<\/h3>\n<p>Table 1 shows the analytical conditions for 5 standard compounds of different saccharides (Fructose, Glucose, Sucrose, Maltose and Lactose). Fig. 1 shows the chromatograms obtained (1000 mg\/L each). It is recommended to wash the column well before use. 5 saccharides eluded within 5 minutes by gradient elution.<\/p>\n<h3>Repeatability<\/h3>\n<p>Table 2 shows the repeatability confirmed by repeated analyses at 250 mg\/L (n=6).<\/p>\n<h3>Linearity<\/h3>\n<p>Fig. 2 shows the calibration curves. Table 3 shows the concentration ranges of the calibration curves. The response of ELSD was plotted on double logarithmic axes because the logarithm of ELSD response is in proportion to the logarithm of concentration. The calibration curves of Fructose and Glucose were created using 6 different concentrations.<\/p>\n<h3>High Speed Analysis of 5 Saccharides in Soft Drinks<\/h3>\n<p>This analysis was carried out under the analytical conditions shown in Table 1. Fig. 3 shows the chromatogram of the soft drink A. Table 4 shows the determination result of saccharides in the soft drink A. The sample was filtered with a 0.2 \u03bcm membrane filter and diluted 20 times with water\/acetonitrile (50:50) for analysis. Fig. 4 shows the chromatogram of the soft drink B. Table 5 shows the determination result of saccharides in the soft drink B. After filtration with a 0.2 \u03bcm membrane filter and 20 times dilution with water\/acetonitrile (50:50), the supernatant obtained was analyzed. 5 saccharides were analyzed without sensitivity adjustment using Wide function of ELSD-LT III. High speed analysis of 5 saccharides in soft drinks was able to be carried out.<\/p>\n<h3>Conclusion<\/h3>\n<p>High speed analysis of monosaccharides and disaccharides was carried out to confirm the separation performance of this method. Determination of monosaccharides and disaccharides in soft drinks was also carried out. 5 saccharides were able to be determined simultaneously without sensitivity adjustment using the Wide function of ELSD-LT III.<\/p>\n<p><em><strong>Nexera is a trademark of Shimadzu Corporation in Japan and\/or other countries.<\/strong><\/em><\/p>\n<\/div><\/section><br \/>\n<div   class='hr hr-default   avia-builder-el-7  el_after_av_textblock  el_before_av_textblock '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock  '   itemprop=\"text\" ><h3>The Authors<\/h3>\n<p>K. Matsuoka, N. Iwata, Shimadzu Corporation, Japan<\/p>\n<\/div><\/section><br \/>\n<div  class='av-social-sharing-box  avia-builder-el-9  el_after_av_textblock  avia-builder-el-last  av-social-sharing-box-minimal '><div class='av-share-box'><h5 class='av-share-link-description av-no-toc '>Share this<\/h5><ul class='av-share-box-list noLightbox'><li class='av-share-link av-social-link-facebook' ><a target=\"_blank\" aria-label=\"Share on Facebook\" href='https:\/\/www.facebook.com\/sharer.php?u=https:\/\/fei-online.com\/high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii\/&#038;t=High%20speed%20analysis%20of%20monosaccharides%20and%20disaccharides%20in%20soft%20drinks%20by%20ELSD-LT%20III' aria-hidden='false' data-av_icon='\ue8f3' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Facebook' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Facebook<\/span><\/a><\/li><li class='av-share-link av-social-link-twitter' ><a target=\"_blank\" aria-label=\"Share on Twitter\" href='https:\/\/twitter.com\/share?text=High%20speed%20analysis%20of%20monosaccharides%20and%20disaccharides%20in%20soft%20drinks%20by%20ELSD-LT%20III&#038;url=https:\/\/fei-online.com\/?p=9645' aria-hidden='false' data-av_icon='\ue8f1' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Twitter' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Twitter<\/span><\/a><\/li><li class='av-share-link av-social-link-whatsapp' ><a target=\"_blank\" aria-label=\"Share on WhatsApp\" href='https:\/\/api.whatsapp.com\/send?text=https:\/\/fei-online.com\/high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii\/' aria-hidden='false' data-av_icon='\uf232' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on WhatsApp' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on WhatsApp<\/span><\/a><\/li><li class='av-share-link av-social-link-linkedin' ><a target=\"_blank\" aria-label=\"Share on LinkedIn\" href='https:\/\/linkedin.com\/shareArticle?mini=true&#038;title=High%20speed%20analysis%20of%20monosaccharides%20and%20disaccharides%20in%20soft%20drinks%20by%20ELSD-LT%20III&#038;url=https:\/\/fei-online.com\/high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii\/' aria-hidden='false' data-av_icon='\ue8fc' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on LinkedIn' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on LinkedIn<\/span><\/a><\/li><li class='av-share-link av-social-link-mail' ><a  aria-label=\"Share by Mail\" href='mailto:?subject=High%20speed%20analysis%20of%20monosaccharides%20and%20disaccharides%20in%20soft%20drinks%20by%20ELSD-LT%20III&#038;body=https:\/\/fei-online.com\/high-speed-analysis-of-monosaccharides-and-disaccharides-in-soft-drinks-by-elsd-lt-iii\/' aria-hidden='false' data-av_icon='\ue805' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share by Mail'><span class='avia_hidden_link_text'>Share by Mail<\/span><\/a><\/li><\/ul><\/div><\/div><\/p><\/div><div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding   avia-builder-el-10  el_after_av_three_fifth  avia-builder-el-last  \" style='border-radius:0px; 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