There are numerous methods for detecting protein interactions and aggregation kinetics, each varying in sensitivity and specificity. Composition-Gradient Multi-Angle Light Scattering (CG-MALS) is an ideal means for measuring proteins in their natural solution: protein samples need not be tagged, immobilised on a surface, or otherwise modified from their natural state.
This sensitive and precise method measures complexes and aggregates through the absolute determination of molar mass and can non-destructively characterise both weak and strong interactions. Simple or complex association stoichiometries may be analysed, providing equilibrium dissociation constants from picomolars to millimolars, as well as self- and cross- viral coefficients and aggregation or association kinetics. The Calypso automated protein association and aggregation system enables the rapid, quantitative, nondestructive characterisation of protein-protein interactions in native solution using CG-MALS. Computer-controlled syringe pumps automate the sample mixing, dilution and delivery, while intuitive software controls the experiment, acquires data and analyses the results. The system can also be used to facilitate the study of the composition dependence of any measurable solution property (spectroscopic, hydrodynamic, calorimetric).
Wyatt Technology Santa Barbara, CA, USA.
Automated protein association and aggregation system